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Ships within 48 hours ยท Estimated delivery Jul 17 - Jul 22
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Description
MIP1b (Macrophage Inflammatory Protein 1 Beta) ELISA Kit (Small Sample)The MIP1b (Macrophage Inflammatory Protein 1 Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB
The MIP1b (Macrophage Inflammatory Protein 1 Beta) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ยฑ 10nm. The concentration of Human MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve.
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