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Description
Human PDIA3 (Protein Disulfide-isomerase A3) ELISA KitThe Human (PDIA3) Protein Disulfide isomerase A3 ELISA Kit measures protein disulfide isomerase A3 in samples. The plate has been pre coated with Human PDIA3 antibody. PDIA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PDIA3 Antibody is added and binds to PDIA3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PDIA3 antibody. After incubation unbound Streptavidin HRP
The Human (PDIA3) Protein Disulfide-isomerase A3 ELISA Kit measures protein disulfide-isomerase A3 in samples. The plate has been pre-coated with Human PDIA3 antibody. PDIA3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PDIA3 Antibody is added and binds to PDIA3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PDIA3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PDIA3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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Exchange/Return Notes
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