DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit
SKU: 67468220162

DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit

Sale price$202.50 Regular price$225.00
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Ships within 48 hours · Estimated delivery Jul 9 - Jul 14

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Description

DHVD3 (1,25-Dihydroxyvitamin D3) ELISA KitThe DHVD3 (1,25 Dihydroxyvitamin D3) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with DHVD3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to DHVD3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The

The DHVD3 (1,25-Dihydroxyvitamin D3) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to DHVD3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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Exchange/Return Notes
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SKU: 67468220162

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