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Description
Rat CATH-B (Cathepsin B) ELISA KitThe Rat (CATH B) Cathepsin B ELISA Kit measures Cathepsin B in Rat samples. The plate has been pre coated with Rat cath B antibody. cath B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat cath B Antibody is added and binds to cath B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated cath B antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Rat (CATH-B) Cathepsin B ELISA Kit measures Cathepsin B in Rat samples. The plate has been pre-coated with Rat cath-B antibody. cath-B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat cath-B Antibody is added and binds to cath-B in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated cath-B antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat cath-B. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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