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Description
EasyStep Rat fT3 (Free Triiodothyronine) ELISA KitThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre coated using protein related techniques are provided separately. Standard Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP conjugated antibody specific to Rat fT3 to each microplate well and
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat fT3 antigen, and the Rat fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ΓβΓΒ±10nm. The concentration of Rat fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.
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